Synthesis and turnover of hdRNA and mRNA in the salivary gland of the blowfly Calliphora erythrocephala

Abstract

We have measured the absolute molar rates of synthesis, accumulation, and turnover of blowfly salivary gland heterodisperse RNA. Twelve- and 84-hr-stage third-instar Calliphora erythrocephala larvae were injected with [ 3H]adenosine, and its flow into glandular ATP, heterodisperse RNA, and polyadenylated RNA was each quantitated over a 360-min time course. The results of these experiments indicate that at least 80% of the newly synthesized heterodisperse RNA mass is a >28 S nuclear species whose average first-order half-life is approximately 20 min. The remaining 20% of the heterodisperse RNA has a 6–28 S size distribution, accumulates in the cytoplasm, and is associated with functional polysomes. The average first-order half-life of this more stable species is 20–25 hr. In addition, we have independently quantitated by optical methods the developmental change in the content of polysome-associated mRNA. The mRNA in these studies also has an average first-order half-life of 25 hr and accounts for 25–55% of the mRNA mass predicted by the incorporation-kinetic analysis of the pulse-labeled heterodisperse RNA. Despite the increased polyteny of the older stage glands, the rates of synthesis and accumulation of each of the individual heterodisperse RNA classes are the same at the 12- and 84-hr stages. Collectively, these results demonstrate that salivary gland functional specialization results from the accumulation of long-lived mRNA and not from changes in the overall rate of mRNA synthesis.