Abstract
Novel base-discriminating fluorescent (BDF) nucleoside, 8-fluorescence-labeled adenosine derivative ( 8Py A ), was developed for the detection of thymine base on a target DNA. The BDF nucleoside was incorporated into oligodeoxynucleotides by post-synthetic modification. BDF probes containing 8Py A selectively emit fluorescence only when the base opposite BDF nucleoside is thymine and act as effective reporter probes for homogeneous SNP typing.
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