Abstract
Abstract New affinity reagents for ATP-dependent enzymes are described. Optimal conditions are evolved for the synthesis of mixed anhydrides of AMP, ADP, ATP with mesitoic acid (MsCOpnA, n = 1−3) and for their 1, N6-etheno, 2′, 3′-dialdehyde and photoactive analogues. UV, CD and fluorescence spectra of the compounds have been analyzed. Hydrolysis of MsCOpnA (n = 1−3) and their etheno analogues over a wide pH range has been carried out.
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