Synthesis and properties of magnetic microspheres based on magnetite and hydrophilic polymers

Abstract

Using emulsification technique, magnetic microspheres (MMS) were prepared from solutions of alginate, pectin, gelatin, chitosan and polyvinyl alcohol. Index of polidispersity of the microspheres was ≤ 0.1. The microspheres from gelatin, chitosan and polyvinyl alcohol covalently crosslinked with glutaraldehyde were resistant to sonication and hydrolysis at room temperature in buffer solution with pH 7.4. The microspheres containing superparamagnetic magnetite particles demonstrated no residual magnetization. After modification, chitosan and polyvinyl alcohol MMS were used for immunomagnetic separation of cells and germs.