Abstract
The ribosomal RNA operon of the archaeon Sulfolobus solfataricus was expressed in vivo in E. coli cells and the fate of the archaeal transcript was analysed both in the presence and in the absence of transcription of the endogenous rRNA operons. The results show that the archaeal transcript is specifically cleaved by the E. coli RNAse III enzyme, yielding 16S and 23S-sized products. The cleavage of the 23S precursor takes place within a helix-bulge-helix motif contained in the double-helical processing stem. However, the maturation of the archaeal rRNAs is not completed, due to their scarce ability to associate with the host ribosomal proteins.
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