Abstract
Development and characterization of hybrid hydrogels loaded with methylene blue, which are designed to apply for photodynamic therapy, are presented. Hybrid hydrogels were synthesized by grafting polyacrylamide onto dextran/dextran sulfate sodium salt using N, N′-methylene-bis-acrylamide as a cross-linker. The differences in microstructure of synthesized hydrogels were proved by scanning electron microscopy. FTIR spectra testify that the chemical nature of hydrogel components affects the hydrogel hydrophilicity. The swelling properties of hydrogels in water and absorption/desorption hydrogels’ ability towards methylene blue were studied. It was shown that dye sorption was dependent on the hydrogel type. The hydrogel based on dextran and polyacrylamide revealed the highest ability to release absorbed dye. The bactericidal effect of this hydrogel loaded with methylene blue and activated by red light in suspension and solid medium of S. aureus was tested. The increase of bactericidal activity of hybrid hydrogel was dependent on radiation doses.
Highlights
In recent years, hydrogels have received increasing attention as a possible means of wound therapy [1]. ese porous networks as new drug carriers for photosensitive drugs have increased research attention because of its water solubility, high stability, and biocompatibility
We focused on the creation and comparative study of the hydrogels with various structures and chemical nature
It was reported that dextran in nonionic form keeps the conformation of macrocoil in the grafting process. is conformation is stabilized by some internal cross-links after the addition of Ce (IV)—the initiator of radical copolymerization dextran with PAA [21]. e mechanism of Ce (IV) initiation involves the formation of chelate complex with OH group of dextran molecule. e decomposing of chelate complex generates free radicals and provokes the grafting of polyacrylamide chains to dextran as well as partial cross-linking of dextran macromolecule
Summary
Hydrogels have received increasing attention as a possible means of wound therapy [1]. ese porous networks as new drug carriers for photosensitive drugs have increased research attention because of its water solubility, high stability, and biocompatibility. Hydrogels have received increasing attention as a possible means of wound therapy [1]. Polysaccharide-based hydrogels have got attraction in drug delivery system and dressing materials due to their extraordinary swelling properties, biodegradability, biocompatibility, and nontoxicity [2]. Hydrogels have been successfully used for antimicrobial drug delivery [3, 4] In this case, reported freeze-dried chitosan/polyvinylpyrrolidone (PVP) hydrogel with the incorporated antibiotic amoxicillin and on carboxymethylcellulose -dextran hydrogel loaded by amphotericin could be useful for treatment of local antifungal infections, with or without concurrent systemic therapy. It was shown that after long-term antimicrobial therapy, skin microbiota become resistant to the antibiotics used during the treatment, as well as to different groups of therapeutics drugs [6]
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