Abstract
Early studies with inhibitors of nucleic acid synthesis resulted in the proposal of a DNA intermediate called a “provirus” in the life cycle of RNA tumor viruses (14). Further studies have provided substantial evidence for the synthesis of proviral DNA (pDNA) upon infection of cells with RNA tumor viruses and a role for the provirus in virus replication and cellular transformation (3,4). The mechanics by which pDNA is synthesized and integrated into the host cell genome, however, remain to be elucidated. There are conflicting reports on the site of pDNA synthesis in an infected cell (5,9), and size and other characteristics of pDNA have not been established. In this report we summarize our results on the synthesis and characteristics of pDNA and the effect of ethidium bromide on integration of pDNA into host cell DNA.
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