Abstract
We have synthesized a sol-gel derived multifunctional silicate based bioactive glass composition[(70-x)SiO2-(30-y-z)CaO-xB2O3-yAg2O-zZrO2,wherein 1<x < 5,0.5<y < 2,0.005<z < 2 mol%] with radiopacity and antibacterial potency, for possible endodontic applications such as vital pulp therapy of paediatric population worldwide with a prevalence of dental caries and primary tooth decay. The composition was physicochemically characterized by X-ray Diffraction (XRD), Field Emission Scanning Electron Microscopy –Energy Dispersive X-ray Analysis (FESEM-EDX), Fourier Transform Infrared Spectroscopy (FTIR), and ThermoGravimetric analysis –Differential Scanning Calorimetry (TG-DSC). The textural properties were measured by Brunauer-Emmett-Teller (BET) surface area measurement and N2 adsorption/desorption study. Next, the in-vitro bioactivity was assessed by soaking the sample in simulated body fluid (SBF).Apart from these, the in-vitro biological properties were assessed by performing cell migration assay, MTT[(3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide] colorimetric assay for cytotoxicity and proliferation assay, matrix mineralization assay on a embryonic fibroblast cell line which can differentiate into other type of cells depending upon the medium. In vitro antibacterial assay was performed on a gram + ve streptococcus.sp. bacterial strain which is the most susceptible oral bacteria and in situ radiopacity was evaluated by taking X-ray digital image and post processed to qualitatively analyze the radiopacity level. In summary the results revealed that the current composition has optimum radiopacity, antibacterial potency and has excellent migration/proliferation capability including calcium mineralization ability that is indicative of its application as a regenerative pulp capping material in endodontic application.
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