Synthesis and in vitro anticancer activity of 4H-pyrano[2,3-d]pyrimidine−1H-1,2,3-triazole hybrid compounds bearing D-glucose moiety with dual EGFR/HER2 inhibitory activity and induced fit docking study

Abstract

• 4 H -Pyrano[2,3- d ]pyrimidine−1,2,3-triazole− d -glucose hybrid compoundssynthesized • Some compounds inhibited for MCF7, HepG2, HeLa cell lines with IC 50 < 4 μM • Compounds 8v,8z,8zc , and 8zf exhibited against EGFR and HER2 tyrosine kinases • Induced fit docking and MM-GBSA simulations for 8zc on EGFR enzyme. The polysubstituted 4 H -pyrano[2,3- d ]pyrimidines 6a-zj containing propargyl group on nitrogen atom 3 of ring have been synthesized by ring-closing reaction of corresponding 2-amino-3-cyano-4 H -pyrans 4a-zj with acetic anhydride and trifluoroacetic acid as catalyst, followed by alkylation with propargyl bromide under ultrasound conditions. A series of 36 analogs of 4 H -pyrano[2,3- d ]pyrimidine 8a-zj bearing d -glucose moiety tethered 1,2,3-triazole derivatives, including eighteen new ones, were synthesized by click chemistry between these N -propargyl derivatives and peracetylated d -glucopyaranosyl azide, with using CuNPs@Montmorillonite as a catalyst under ultrasound condition in the presence of DIPEA in t -BuOH/H 2 O at 25°C for 20 min. These compounds exhibited potent anticancer activity against tested cancer cells, including MCF-7, HepG2, and HeLa. Amongst tested compounds, some compounds exhibited strong activity against tested cancer cell lines with IC 50 < 4 μM, such as 8v,8x,8z,8zc,8zf and 8zg against MCF-7, 8s,8t,8w,8zh , and 8zi against HepG2, 8h,8j,8zf and 8zh against HeLa cancer cell lines. Compounds 8v,8z,8zc , and 8zf exhibited remarkable inhibitory activity against EGFR and HER2 tyrosine kinases in comparison with Lapatinib. The resulted docking studies showed that compound 8zc displayed binding mode like Erlotinib on EGFR enzyme and showed good binding energies. Induced fit docking, MM-GBSA calculation, and molecular dynamics simulations were carried out to elucidate the inhibitory potential of compound 8zc against tested enzyme 4HJO. Docking analysis showed that amongst the hydrophilic and hydrophobic interactions at the active site of EGFR enzyme, the hydrogen-bond bindings between acetate function and appropriate amino acid residues displayed the most important contribution in intensifying their potency against this enzyme.