Synthesis and Identification of New Heterocyclic Compounds from Isatin and Study their Inhibition Ability of Colorectal Adenocarcinoma

Abstract

Heterocyclic compounds showed a wide range of applications but in medicinal chemistry are of particular interest. Heterocyclic were the active compounds in many natural drugs also some are still used today, for instance, quinine derivatives and morphine. Colorectal carcinoma is the world's third most common neoplasm and the second leading cause of cancer-related death. This research aims to identify a prospective medication with a high affinity for treating colorectal cancer. A Series of heterocyclic compounds 1, 2, 3, and 4 were synthesized and confirmed through the use of different spectroscopic techniques (IR and NMR). The in vitro their anticancer potential by means of cytotoxicity and selectivity determination of the compounds 1a, 2b, 3a, and 4a against HT-29 and MA104 were assessed. The experimental Isatin derivative 2b has a reasonable and strong antineoplastic effect on colorectal cancer HT-29 cell line, as evidenced by its significant decrease in cellular viability and increase affinity, according to our findings. In this study, 2b also showed considerable safety qualities based on the parameters tested on the Vero cell line.