Abstract
Infections caused by multidrug-resistant bacteria such as P. aeruginosa are important therapeutic complications. Piperacillin/Tazobactam is considered a safe antimicrobial agent. But we should not ignore the prevalence of resistant strains to this drug. In this work, a new polymeric micelle composed of Piperacillin/Tazobactam-loaded Poly (ethylene glycol) methyl ether-block-poly (lactide-co-glycolide) (PLGA-PEG) was developed to improve the antimicrobial performance of P/T. The SEM and TEM studies of PLGA-PEG micelle showed, semi-spherical morphology with a mean diameter of below 30 nm. Zeta potential results indicated that the surface charge of PLGA-PEG micelle was −2.98 mV, while after encapsulation of P/T, the surface charge decreases to −4.13 mV. Clinical strains of P. aeruginosa were isolated and their resistance pattern against different antibiotics was evaluated. The MIC of free and P/T -Loaded PLGA-PEG micelles was determined. Also, the effect of free or P/T micelle against minimal biofilm eradication concentration and motility inhibition was evaluated. The bacterial isolates were resistant to most common antibiotics. The MIC of the free drug form and micelle form ranged from 4 to 512 µg/ml and 2 to 256 µg/ml, respectively. Generally, micelle showed more effective antibiofilm activities, inhibition of bacterial motility and reducing the MIC than that free drug form.
Highlights
Pseudomonas aeruginosa is a Gram-negative, rod-shaped bacteria with the ability to survive in a variety of environmental conditions
The chemical structure of the PLGA-PEG copolymers was studied with 1 H-NMR and 13 C-NMR by integrating the signals related to each monomer (Figures 1 and 2)
We developed a micellar nanocarrier based formulation that increases the effectiveness of the Piperacillin/ Tazobactam
Summary
Pseudomonas aeruginosa is a Gram-negative, rod-shaped bacteria with the ability to survive in a variety of environmental conditions. Pseudomonas infections are acquired from the hospital environment. This microorganism is an important nosocomial pathogen (Fujii et al, 2014). Among infections caused by gram-negative bacilli, P. aeruginosa plays an important role especially in patients with severe illness and in immunocompromised patients. Control of infections caused by this bacterium is critical. Physicians have encountered major challenges in the treatment of infections caused by gram-negative pathogens, especially Pseudomonas infections (Pena et al, 2009; El Zowalaty et al, 2015; Sligl et al, 2015)
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