Synthesis and Evaluation of Organic–Inorganic Hybrid Molecularly Imprinted Monolith Column for Selective Recognition of Acephate and Phosphamidon in Vegetables

Abstract

ABSTRACTIn this study, a molecularly imprinted capillary monolithic column that can recognize the acephate and phosphamidon was synthesized by the molecular imprinting technique combined with a nonhydrolytic sol–gel process using intermediate 4‐(dimethoxyphosphorothioylamino)butanoic acid as the template molecule. The resulting material was characterized by scanning electron microscopy and Fourier transform infrared spectroscopy. The potential of the prepared imprinted monolithic columns used as the stationary phase for capillary electrochromatography (CEC) was evaluated. The effects of operating voltage, pH, and acetonitrile content on the electroosmotic flow of the imprinted monolithic columns were evaluated in CEC. Under optimal conditions, the cucumber and lettuce samples spiked with acephate and phosphamidon were extracted and analyzed by this method with satisfactory recoveries ranging from 79.7 to 94.4%. The relative standard deviation for five replicates of 0.1 mg/L mixed solution of acephate and phosphamidon was in the range of 2.7–4.6%.