Synthesis and Evaluation of Cy7-Rituximab Targeting CD20 Antigen for in vivo Animal Fluorescence Imaging

Abstract

A new fluorescence molecular imaging probe Cy7-Rituximab was synthesized through the connection of fluorescence Cy7 dye and protein Rituximab.The probe was fully characterized by fluorescence spectrophotometer,SDS-PAGE and confocal microscopy.The uptake of Cy7-Rituximab probe in DLBCL cells was observed by laser scanning confocal microscope.Cy7-Rituximab probe of 0.4 mL(0.6 mg / mL) was injected to BALB / C nude mice through tail vein.The biodistribution of the novel probe Cy7-Rituximab was eventually observed,especially its specific concentration in spleen.