Synthesis and dissolution behavior of nanosized silicon and magnesium co-doped fluorapatite obtained by high energy ball milling

Abstract

Nanosized hydroxyapatite (HA) powders exhibit a greater surface area than coarser crystals and are expected to show an improved bioactivity. In addition, properties of HA can be tailored over a wide range by incorporating different ions into HA lattice. The aim of this study was to prepare and characterize silicon and magnesium co-doped fluorapatite (Si–Mg–FA) with a chemical composition of Ca9.5Mg0.5 (PO4)5.5(SiO4)0.5F2 by the high-energy ball milling method. Characterization techniques such as X-ray diffraction analysis (XRD), Fourier transformed infrared spectroscopy (FTIR), energy dispersive X-ray spectroscopy (EDX) and transmission electron microscopy (TEM) were utilized to investigate the structural properties of the obtained powders. Dissolution behavior was evaluated in simulated body fluid (SBF) and physiological normal saline solution at 37°C for up to 28 days. The results of XRD and FTIR showed that nanocrystalline single-phase Si–Mg–FA powders were synthesized after 12h of milling. In addition, incorporation of magnesium and silicon into fluorapatite lattice decreased the crystallite size from 53nm to 40nm and increased the lattice strain from 0.220% to 0.296%. Dissolution studies revealed that Si–Mg–FA in comparison to fluorapatite (FA), releases more Ca, P and Mg ions into SBF during immersion. 175ppm Ca, 33.5ppm P and 48ppm Mg were detected in the SBF containing Si–Mg–FA after 7days of immersion, while for FA, it was 75ppm Ca, 21.5ppm P and 29ppm Mg. Release of these ions could improve the bioactivity of the obtained nanopowder. It could be concluded that the prepared nanopowders have structural properties such as crystallite size (~40nm), crystallinity degree (~40%) and chemical composition similar to biological apatite. Therefore, prepared Si–Mg–FA nanopowders are expected to be appropriate candidates for bone substitution materials and also as a phase in polymer or ceramic-based composites for bone regeneration in tissue engineering applications.