Abstract
This work aimed to investigate the process parameters of phytosterol esters (PE) synthesis catalyzed by copper oxide-nano hydroxyapatite (CuO-NHAP) and the crystallization purification technology. CuO-NHAP was prepared. The synthesis of PE catalyzed by CuO-NHAP was performed. After Response Surface Methodology optimization, the optimal process parameters were as follows: reaction temperature, 180 oC; reaction time, 6.3 h; acid-alcohol molar ratio, 1.9; catalyst amount, 1.05%. Under these conditions, the esterification rate of phytosterols was 99.8%. The crystallization purification technology of esterification product was explored. The optimal crystallization conditions were as follows: crystallization solvent, octanoic acid; crystallization temperature, 0 oC; solvent-product molar ratio, 4: 1; crystallization time, 10 h. Under these conditions, the unreacted fatty acids and other impurities were affectively removed, and the PE purity in purification product was 98.2%. These synthesis and crystallization purification technologies of PE are relatively green, safe, efficient and economic, and have the potential to be applied to the industrial preparation of PE product.
Highlights
Phytosterols are the sterols with similar structure and biochemical characteristics to cholesterols
CuO-nano hydroxyapatite (NHAP) was used to catalyze the synthesis of phytosterol esters (PE)
Based on the results determined by single-factor experiments in our previous study [20], the process parameters were further optimized by Response Surface Methodology (RSM)
Summary
Phytosterols are the sterols with similar structure and biochemical characteristics to cholesterols They have high medicinal value, and can effectively reduce the concentration of neutral triglycerides and cholesterol in blood, preventing and treating the cardiovascular diseases (de Jong et al, 2008; Cofán & Ros, 2015; Plat et al, 2019). The synthesis of phytosterol esters (PE) can be completed by enzymatic catalysis. This method has the advantages of mild conditions and good product quality. The process parameters of PE synthesis catalyzed by CuO-NHAP were optimized by surface methodology. The objective was to provide a basis for preparation of PE for food industry application
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
