Synthesis and correction of albumin magnetic nanoparticles with organic compounds for absorbing and releasing doxorubicin hydrochloride

Abstract

In an ideal delivery system, carrier nanoparticles are used as a promising alternative with minimized adverse effects to treat a variety of diseases. The purpose of this study was to create a targeted delivery system for doxorubicin hydrochloride (DOX−HCl), using Fe3O4-L@HSA-β-cyclodextrin (β-CD)/Allyl amine nanoparticles. In this study, magnetite nanoparticles (Fe3O4) were produced by co-precipitation, while albumin nanoparticles (HSA) were produced by the desolvation method. The properties of the nanoparticles were studied by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and vibrating sample magnetometer (VSM). To evaluate the loading of the drug on the synthesized nanoparticles, ultraviolet-visible spectrophotometry (UV–Vis) and atomic force microscopy (AFM) were used. DOX−HCl loading was studied by two experiments: an optimization method (OM) and a one-factor-at-a-time method (OFATM). Response surface methodology (RSM) was utilized to optimize the parameters. The optimal conditions for drug loading of nanoparticles in OM and OFATM methods were 81.46% and 77%, respectively. The release of DOX−HCl drug from the synthesized nanoparticles at a temperature of 37 °C and specific time in pH 5.3 and pH 7.4 was 83.35% and 38.39%, respectively. To examine the cytotoxicity of nanoparticles with drugs, the MTT assay was performed using MCF-7 cancer cells. Finally, cell uptake was tested using inductively coupled plasma-mass spectrometry (ICP-MS).