Abstract

Silver nanoparticles were synthesized using Olive oil (O-AgNPs) as reducing as well as capping agent and extensively characterized by UV–vis spectroscopy, fourier transform infrared, energy disperse spectroscopy, dynamic light scattering and atomic force microscopy. The chrome yellow color solution of O-AgNPs show the typical absorption maximum at 430nm. FTIR analysis revealed that the carbonyl (CO) groups of Olive oil plays the most vital role in reduction of Ag+ and the nanoparticles synthesis. The morphology of O-AgNPs were found spherical in nature, while the size of O-AgNPs ranges between 35 to 65nm as established by AFM and DLS studies, respectively. Stability of O-AgNPs studied by varying storage period, temperature, salt and pH of the medium were found quite stable probably because of oil suspension nature. Despite potent antimicrobial, anti-biofilm and biofilm eradicating activities, O-AgNPs were found to be non-toxic on cell lines. Moreover, chemosensing properties of the O-AgNPs were also tested against different drugs. The O-AgNPs showed high selectivity towards nitrofurazone (NFZ) with the lowest detection limit of 1.88μM as was monitored by UV–vis spectroscopy. The mechanism of O-AgNPs interaction with drug was followed by DLS suggest that NFZ induces a time dependent nanoparticles aggregation.

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