Synthesis and characterization of thermosensitive poly(N-isopropylacrylamide-co-hydroxyethylacrylamide) microgels as potential carriers for drug delivery

Abstract

Thermoresponsive colloidal microgels were prepared by precipitation copolymerization of N-isopropylacrylamide (NIPAM) and N-hydroxyethylacrylamide (HEAM) with various concentrations of a cross-linker in the presence of an anionic surfactant, sodium dodecylsulphate (SDS). The volume phase transition temperature (VPTT) of the prepared microgels was studied by dynamic light scattering (DLS), ultraviolet–visible spectroscopy (UV–vis) and proton nuclear magnetic resonance (1H-NMR) spectroscopy. In addition, atomic force microscopy (AFM) was used to characterize the polydispersity and morphology of the microgels. Results indicated that poly(NIPAM-co-HEAM) microgels are spherical and monodisperse. VPTTs of microgels determined by DLS and UV–vis methods are almost the same and very close to the human body temperature, presenting the microgels as candidates for biomedical application. The temperature at which the phase transition occurred is nearly independent of the cross-linking density, whereas the transition range is deeply influenced by temperature. Also, the SDS concentration was increased to decrease the average hydrodynamic size of the microgels, due to the electrostatic repulsion between the charged particles during the polymerization process. 1H-NMR spectra of the microgels show a decrease in peak intensity with an increased temperature due to a reduction in molecular mobility of the polymer segments. Release rates of propranolol from microgels are deeply influenced by temperature; below the VPTT at 25 °C, the drug is rapidly released at a rate comparable to that of a free drug, whereas above the VPTT (37 and 42 °C), a fraction of the drug is mechanically expulsed in the first five min, followed by a prolonged release.