Synthesis and Characterization of Silver-Strontium (Ag-Sr)-Doped Mesoporous Bioactive Glass Nanoparticles.

Shaher Bano,Muhammad Yasir,Akbar Niaz,Abdul Wadood,Muhammad Atiq Ur Rehman,Muhammad Salman Maqbool,Memoona Akhtar

doi:10.3390/gels7020034

Abstract

Biomedical implants are the need of this era due to the increase in number of accidents and follow-up surgeries. Different types of bone diseases such as osteoarthritis, osteomalacia, bone cancer, etc., are increasing globally. Mesoporous bioactive glass nanoparticles (MBGNs) are used in biomedical devices due to their osteointegration and bioactive properties. In this study, silver (Ag)- and strontium (Sr)-doped mesoporous bioactive glass nanoparticles (Ag-Sr MBGNs) were prepared by a modified Stöber process. In this method, Ag+ and Sr2+ were co-substituted in pure MBGNs to harvest the antibacterial properties of Ag ions, as well as pro-osteogenic potential of Sr2 ions. The effect of the two-ion concentration on morphology, surface charge, composition, antibacterial ability, and in-vitro bioactivity was studied. Scanning electron microscopy (SEM), X-Ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR) confirmed the doping of Sr and Ag in MBGNs. SEM and EDX analysis confirmed the spherical morphology and typical composition of MBGNs, respectively. The Ag-Sr MBGNs showed a strong antibacterial effect against Staphylococcus carnosus and Escherichia coli bacteria determined via turbidity and disc diffusion method. Moreover, the synthesized Ag-Sr MBGNs develop apatite-like crystals upon immersion in simulated body fluid (SBF), which suggested that the addition of Sr improved in vitro bioactivity. The Ag-Sr MBGNs synthesized in this study can be used for the preparation of scaffolds or as a filler material in the composite coatings for bone tissue engineering.

Highlights

Millions of medical devices are being implanted nowadays in patients related to bone diseases and accidental surgeries, thanks to the advancement of biomaterials
The morphology of the synthesized mesoporous bioactive glass nanoparticles (MBGNs) was investigated by Scanning electron microscopy (SEM) analysis
In this study, we developed MBGNs doped with Ag in its pure form (XRD results indicate no crystalline peak of silver oxide), due to which it was possible to release silver in an ionic form rather than particulate form [10]

Summary

Introduction

Millions of medical devices are being implanted nowadays in patients related to bone diseases and accidental surgeries, thanks to the advancement of biomaterials. Bioactive glasses (BGs), new generation of bio-ceramics, are preferred biomaterials in a wide range of biomedical applications such as the regeneration of hard tissues (bones and drug delivery), owing to their bioactivity (in vitro and in vivo), osteoconductivity, osteoinductivity, high degradation rate, tailorable morphology (e.g., miniature size, high specific surface area, pore structure), and favorable physicochemical properties (e.g., bone bonding potential, biodegradability) [5,6,7]. Composition, pore size, particle size, and the specific surface area are the main factors on which the bioactivity and degradation rate strongly depends. These required characteristics can be obtained by employing mesoporous bioactive glass nanoparticles (MBGNs)

Methods

Results

Conclusion