Synthesis and Characterization of Photoluminescent Ce(III) and Ce(IV) Substituted Hydroxyapatite Nanomaterials by Co-Precipitation Method: Cytotoxicity and Biocompatibility Evaluation.

Andrei Viorel Paduraru,Florin Iordache,Bogdan Stefan Vasile,Roxana Trusca,Ecaterina Andronescu,Ovidiu Cristian Oprea,Adina Magdalena Musuc

doi:10.3390/nano11081911

Abstract

Improved compounds of Ce(III) and Ce(IV)-doped hydroxyapatite (Ca10-xCex(PO4)6(OH)2) with different concentrations such as x = 0.5, 1, 2.5, 5, and 10%, obtained by the simple co-precipitation method were synthesized. The cerium (3+) and cerium (4+)-doped hydroxyapatite were evaluated for biocompatibility and fluorescence properties. It was found that the cerium-HAp powders were non-toxic, even at higher level of concentration. The synthesized powders were further characterized by FTIR spectrometry, UV-Vis spectroscopy, XRD diffraction, SEM and TEM analysis. Therefore, the present study proves that the developed cerium (3+) and cerium (4+)-doped hydroxyapatite, respectively can be widely used as luminescent labeling materials, with improved biological properties.

Highlights

In the world of bioceramics, chemical compounds such as calcium apatites and other calcium orthophosphates are widely used in many fields of science, including geology, chemistry, biology, and medicine due to their abundance and presence in living organisms [1]
The main group of bioceramics is derived from calcium phosphate (CaP), the others are zirconium (ZrO2 ), alumina (Al2 O3 ), carbon, silicates, and phosphate ceramics [2]
The main goal of this study is to demonstrate the application of the obtained cerium(III)

Summary

Introduction

In the world of bioceramics, chemical compounds such as calcium apatites and other calcium orthophosphates are widely used in many fields of science, including geology, chemistry, biology, and medicine due to their abundance and presence in living organisms [1]. Bioceramics can be defined as a biocompatible ceramic material, manufactured in a porous, dense, amorphous or crystalline form, which can be used for the repair and reconstruction of different parts of tissue. The main group of bioceramics is derived from calcium phosphate (CaP), the others are zirconium (ZrO2 ), alumina (Al2 O3 ), carbon, silicates, and phosphate ceramics [2]. Various studies used nanoparticles or different components for this purpose [3]. Hydroxapatite (HAp) is the main inorganic compound of human hard tissue [4]. An important application of HAp is in the treatment with chemotherapeutic drugs and antibiotics, mainly due to its porous surface and highly biodegradable properties [7]. Various studies suggest that calcium phosphate nanoparticles can be successfully used as a fluorescent probe

Objectives

Methods

Results

Conclusion