Abstract
In this study, a 2′-O,4′-C-methyleneoxy-bridged 5-methyluridine with three consecutive acetal groups (TaNA with a thymine base) was synthesized. The synthesized TaNA was incorporated into oligonucleotides (ONs), and their properties were compared with those of ONs containing the 7′-methylated analog (Me-TaNA) modifications previously reported by us. The duplex stability of TaNA with single-stranded RNA was comparable to that of Me-TaNA. However, the triplex stability of TaNA-modified ONs with double-stranded DNA was slightly lower than that of Me-TaNA-modified ONs. Additionally, the enzymatic stability of TaNA-modified ONs was lower than that of Me-TaNA-modified ONs. These results suggest that the 7′-methyl substitution on the TaNA structure is crucial for enhancing the properties of modified ONs.
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