Synthesis and characterization of novel trimethyltin(IV) and tributylltin(IV) complexes of anticoagulant, WARFARIN: Potential DNA binding and plasmid cleaving agents

Abstract

Novel trimethyltin(IV) (1) and tributyltin(IV) (2) derivatives of an anticoagulant drug warfarin (WR) have been synthesized and characterized through elemental analysis, FTIR studies, multinuclear NMR and ESI mass spectrometry and DFT calculation. Their DNA binding profile (mode and extent of binding with CT DNA) through UV–visible and fluorescence spectrophotometric titrations reveal their partial intercalation inside the base pairs of DNA, and the binding constant (Kb) calculated through UV–visible titration are in the order of 104 M−1. Their partial intercalation has also been validated through a decrease in the viscosity of CT DNA with increasing the complex concentration. Both the complexes are potential concentration dependent plasmid cleaving agent which has been confirmed though gel electrophoresis of pBR322 plasmid. Both the organotin(IV) complexes have been found to exhibit a greater potential towards DNA binding and fragmentation in comparison to WR.