Abstract
In this research, we try to report a new biocompatible nanocarrier that is synthesized by chemical modification of magnetic inulin through interaction with acetylated choline chloride. Further modifications were made to the synthesized nanocomposite using chitosan biopolymer. The magnetic inulin nanocarrier's structure was characterized with FT-IR, NMR, DLS, zeta potential, XRD, TEM, and FESEM techniques. The biological properties of the nanocarrier were investigated in gene delivery to HEK-293T cells. The nanocarrier's loading capacity and in vitro release were evaluated by gel retardation and the dialysis method. Plasmid transfection efficiency was optimal at N/P ratio 2. Chemically modified magnetic inulin did not show any intrinsic toxicity toward cells. The results show that nanoparticles in physiological body pH (7.4) can preserve and protect plasmid DNA with low toxicity and can be used as a non-viral carrier for gene transfer and delivery with slow release for cancer treatment.
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