Abstract
This study describes an eco-friendly, rapid method for green synthesis of silver nanoparticles (Ag NPs) from an aqueous solution of silver nitrate using Pinus densiflora for. multicaulis Uyeki young cone extract in a single-pot process. Color changes, ultraviolet–visible spectra (444.5nm), X-ray diffraction peaks (2θ=39.68, 46.92, 68.12, and 79.10), and Fourier transform infrared spectroscopy (FT-IR) confirmed the presence of Ag NPs and phytochemicals. Transmission electron microscopy showed that the nanoparticles were mostly oval in shape, with a few triangular-shaped particles. Average particle size was 30–80nm. Phytochemicals present in the young pine cone extract were likely responsible for the reduction of Ag+ ions. The synthesized Ag NPs (40μg) had a 7mm larger zone of inhibition against the skin pathogen Brevibacterium linens than commercial Ag NPs, Propionibacterium acnes (14mm), Bacillus cereus (9mm) and Staphylococcus epidermidis (10mm).
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