Synthesis and characterization of multilayered nanobiohybrid magnetic particles for biomedical applications

Abstract

In this study, new magnetic nanobiohybrid particles with a multilayered structure were prepared. 7±1nm oleic acid modified magnetite nanoparticles (MNP) were synthesized via the chemical coprecipitation method. Then, magnetite nanoparticles clusters were coated with a thin silica shell (MS) by the sol-gel approach. The as-synthesized MS particles have a spherical shape and an average size of 147±20nm according to SEM images. Then, bovine serum albumin was conjugated 9–10wt% to aminated particles through glutaraldehyde activation (BSA-MS). BSA-MS particles were then compared with MS particles as a platform for precipitation of subsequent protein shell which was imprinted by desolvation method (BSA shell-BSA-MS & BSA shell-MS particles, respectively). TGA data revealed that an albumin shell of about 17 and 31wt% has been formed on MS and BSA-MS particles, respectively. VSM analysis results indicated the superparamagnetic behavior for magnetite, MS, and BSA shell-BSA-MS particles. Cellular toxicity of MS and BSA shell-BSA-MS particles was also investigated on HepG2 cells. The presence of albumin as a biomolecule coating on the surface of MS particles showed an improving effect to reduce the cytotoxicity. The properties of the designed particles propose the multilayered nanobiohybrid magnetic particles as a promising candidate for multifunctional biomedical applications.