Abstract
A new method for the isolation and enrichment of ofloxacin enantiomers from fish samples was developed using magnetic molecularly imprinted polymers (MMIPs). These polymers can be easily collected and rapidly separated using an external magnetic field, and also exhibit a high specific recognition for ofloxacin enantiomers. The preparation of amino-functionalized MMIPs was carried out via suspension polymerization and a ring-opening reaction using rac-ofloxacin as a template, ethylenediamine as an active group, glycidyl methacrylate and methyl methacrylate as functional monomers, divinylbenzene as a cross-linker, and Fe3O4 nanoparticles as magnetic cores. The characteristics of the MMIPs were assessed using transmission electron microscopy (TEM), X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), and vibrating sample magnetometer (VSM) measurements. Furthermore, the adsorption properties were determined using Langmuir and Freundlich isotherm models. The conditions for use of these MMIPs as magnetic solid-phase extraction (MSPE) sorbents, including pH, adsorption time, desorption time, and eluent, were investigated in detail. An extraction method using MMIPs coupled with high performance liquid chromatography (HPLC) was developed for the determination of ofloxacin enantiomers in fish samples. The limits of quantitation (LOQ) for the developed method were 0.059 and 0.067 μg∙mL−1 for levofloxacin and dextrofloxacin, respectively. The recovery of ofloxacin enantiomers ranged from 79.2% ± 5.6% to 84.4% ± 4.6% and ofloxacin enantiomers had good linear relationships within the concentration range of 0.25–5.0 μg∙mL−1 (R2 > 0.999). The obtained results demonstrate that MSPE-HPLC is a promising approach for preconcentration, purification, and simultaneous separation of ofloxacin enantiomers in biomatrix samples.
Highlights
In recent years, the differences in the pharmacology and pharmokinetics of enantiomers of chiral drugs have received increasing attention [1,2]
molecularly imprinted polymers (MMIPs) were synthesized by suspension polymerization
The MMIPs were synthesized by suspension polymerization according to the literature procedure [32,33,34,35] with a minor modification
Summary
The differences in the pharmacology and pharmokinetics of enantiomers of chiral drugs have received increasing attention [1,2]. Efficient, and fast pretreatment strategies using molecular imprinting techniques [15,16] or magnetic solid-phase extraction (MSPE) [17,18,19,20] have received more attention. New amino-functionalized MMIPs as MSPE sorbents were prepared for the extraction of OFL enantiomers in fish samples. A method based on MSPE coupled with chiral-HPLC analysis was successfully optimized for the separation and determination of OFL enantiomers in fish samples. This proposed technique was shown to be a reliable and effective analytical method for determination of trace amounts of chiral drugs in biomatrix samples. Enantiopure templates can be used for the preparation of imprinted polymers with enantiodiscriminative features that could be successfully applied for enantioseparation and drug delivery in the future [26,27]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
