Abstract
With the help of the solid phase peptide synthesizing method, we synthesized Kalata B2 cyclotide and the final product was purified by preparative HPLC. The sequence of Kalata B2 is GLPVCGETCFGGTCNTPGCSCTWPICTRD and its m/z M + H mass is 2955.53. It had been first isolated from Oldenlandia affinis plant leaves extract which belongs to Rubiaceae family. The synthesis of Kalata B2 has been characterized through UPLC/MS, LC/MS, CD, 1HNMR, and IR.
Highlights
Cyclotides are a unique and growing family of head-to-tail cyclized peptides that contain six cysteine residues oxidized into three disulfide bonds built a cystine knot motif (CCK) [1]
We report the synthesis of Kalata B2 by using chemical synthesizing method and it is solid phase peptide synthesis (SPPS)
Synthesis of Kalata B2 cyclotide has been achieved by manual SPPS method
Summary
Cyclotides are a unique and growing family of head-to-tail cyclized peptides that contain six cysteine residues oxidized into three disulfide bonds built a cystine knot motif (CCK) [1]. Kalata B2 is a cyclotide and cyclotide is one of the types of cyclopeptides in which two main forms of cyclic bonds are present These two main forms of peptide cyclization are: 1) Cyclization by the formation of the amide bond between the N-terminal and the C-terminal amino acid residues (head-to-tail cyclization) [3] and 2) cyclization involving the side chains (S-S bond) of individual amino acids (backbone to backbone cyclization) [4]. Rajasekharan defense against pests and pathogens, cyclotides act as exceptionally stable with unique structurally. They occur in several species of plant families such as Rubiaceae, Violaceae, Cucurbitaceae, Fabaceae, and Solanaceae with an individual plant producing up to hundreds of different cyclotides [5].
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