Synthesis and Characterization of Indole Azo Metal Complexes and Study of their Biological Activity

Abstract

Novel azo dye ligand APAI namely 3-(4-(antipyrinyl)azo)-indole was synthesized by the coupling of diazonium salt of 4-aminoantipyrine and indole compound in basic conditions. Co(II) and Zn(II) complexes were synthesized in 1:2 molar ratio of metal ion to ligand. The structure of the APAI ligand and the metal complexes was established using 1H-NMR, IR, mass spectrometry, UV-Vis, powdered XRD, molar conductivity and magnetic susceptibility. The APAI ligand exhibited significant photochromic characteristics as well as high acidity sensing capability. The infrared data indicated that APAI dye behaves as bidentate ligand. The spectral studies indicated to octahedral geometry for Co(II) and Zn(II) complexes. The cytotoxicity impact of APAI ligand and zinc complex was screened against the MCF-7 cell line (human breast cancer) and health cells of HdFn. The MTT results indicated that APAI ligand and zinc complex induce high cytotoxicity potential against MCF-7 cells with low toxicity against healthy cells of HdFn. The APAI ligand induces higher cytotoxicity potential against MCF-7 cells as compared to zinc complex.