Synthesis and characterization of human bone-like hydroxyapatite using Schiff's base

Abstract

Hydroxyapatite (HAp) has prospective applications in the field of biomaterials as it has good biocompatibility and mechanical reliability. HAp has been synthesized using surfactants and organic modifiers, which follow the mechanism of chelating the calcium and phosphate precursors to form the calcium phosphate mineral mimicking the mineral in native human bone. The method followed in this work exploits schiff’s base which follows the same mechanism of chelating the reactants necessary for HAp formation. Needle-like nanostructures were formed in the presence of the schiff’s base derived from salicylaldehyde and 1, 4-diaminobutane. The synthesized schiff’s base and HAp were characterized using FT-NMR, XRD and FT-IR to confirm their chemical structure and crystallinity. The cytotoxic evaluation of the synthesized HAp was performed using MTT assay with murine fibroblast (L929) cells, which proved the proliferation activity of the cells in presence of HAp was found to be directly proportional to the time of exposure and HAp concentration, hence, inferring that the synthesized HAp is not only biocompatible but also promotes cell proliferation.