Synthesis and Characterization of Exopolysaccharide Encapsulated PCL/Gelatin Skin Substitute for Full-Thickness Wound Regeneration.

Ahmad Hivechi,Masoud Hamidi,Kaveh Ebrahimzadeh,Alireza Rezapour,Peiman Brouki Milan,Amir Reza Gholipour,Cédric Delattre,Naser Amini,Khashayar Modabberi,Moein Amoupour,Faezeh Sedighi,S Hajir Bahrami

doi:10.3390/polym13060854

Abstract

Loss of skin integrity can lead to serious problems and even death. In this study, for the first time, the effect of exopolysaccharide (EPS) produced by cold-adapted yeast R. mucilaginosa sp. GUMS16 on a full-thickness wound in rats was evaluated. The GUMS16 strain’s EPS was precipitated by adding cold ethanol and then lyophilized. Afterward, the EPS with polycaprolactone (PCL) and gelatin was fabricated into nanofibers with two single-needle and double-needle procedures. The rats’ full-thickness wounds were treated with nanofibers and Hematoxylin and eosin (H&E) and Masson’s Trichrome staining was done for studying the wound healing in rats. Obtained results from SEM, DLS, FTIR, and TGA showed that EPS has a carbohydrate chemical structure with an average diameter of 40 nm. Cell viability assessments showed that the 2% EPS loaded sample exhibits the highest cell activity. Moreover, in vivo implantation of nanofiber webs on the full-thickness wound on rat models displayed a faster healing rate when EPS was loaded into a nanofiber. These results suggest that the produced EPS can be used for skin tissue engineering applications.

Highlights

The skin, the largest organ in the body, it protects interior organs against toxins and environmental microorganisms and prevents dehydration in non-aquatic animals
EPSs isolated from R. mucilaginosa sp
GUMS16 was used for skin regeneration investigation

Summary

Introduction

The skin, the largest organ in the body, it protects interior organs against toxins and environmental microorganisms and prevents dehydration in non-aquatic animals. Skin accounts for about 8% of body weight, which varies in thickness from 1.5 mm to 4 mm and depends on the age of the individual and the area of the body. The skin is a complex organ with many essential functions; for instance, it acts as a mechanical barrier, participates in the body’s thermal regulation, helps initiate immunological processes, is involved in the production of melanin, and protects the body against UV radiation [2]. To accomplish all these functions, the skin has many different structures and contains diverse cells with different properties

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Conclusion