Abstract
Copper oxide (CuO) nanoparticles (NPs) were synthesized biological methods using aqueous extract of leaves of Morus, Citrus, and Gossypium. Leaves as a reducing and capping agent. The advantages of this methods are simple operation, use of cheap, natural, nontoxic and benign precursors, absence of toxic reagents and mild and environmentally friendly conditions. The green synthesized CuO NPs was characterized by X-ray diffraction (XRD) analysis demonstrated that the CuO NPs were crystalline with a mono clinic structure. The formation of NPs is monitored by recording the UV –vis optical properties exhibits in UV - region and band gap energy (Eg) greater than 3 eV. Electrical conductivity increases when temperature increases during the synthesis. Antibacterial study of the CuNPs was established using both gram positive and gram negative pathogens. 
 Keywords :Biological methods, CuO nanoparticles, Extract, Antibacterial.
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