Synthesis and characterization of bioactive silver nanoparticles from red marine macroalgae Chondrococcus Hornemannii

Abstract

Nanobiotechnology is developing as a rapidly growing area with its various science and technology applications to produce novel substances at the nanoscale level (1–100 nm). Seaweed extracts have been recently used to synthesize silver nanoparticles; meanwhile, they have abundant bioactive compounds. In this current research, we have described the synthesis of silver nanoparticles (AgNPs) by reducing the silver ions current in silver nitrate solution by using the aqueous seaweed extract of Chondrococcus hornemannii. The synthesized nanoparticles were characterized by UV, FTIR, XRD, and Zeta potential. The antibacterial activity of biosynthesized nanoparticle produced Chondrococcus hornemanniiseaweed aqueous extract was explored against various pathogenic bacteria like Streptococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. The biosynthesized AgNPs exhibited a robust antioxidant activity of 94% at 100 μg/mL and strong hemolytic action 81% at 100 μg/mL. The AgNPs were tested against different bacterial pathogens showed antibacterial activity. The green synthesized silver nanoparticle showed significant cell cytotoxicity effects on U937 cell lines at 20 µg/mL. Finally, the AgNPs induced differential cytokine and chemokine expression profiling in vitro. Accordingly, the green biosynthesized AgNPs exhibited excellent antibacterial activity, antioxidant activity, hemolytic activity and increased cytokine, and chemokine expression level with an initiative to have prospective for nano-based drug delivery system.