Abstract

A series of eight new (E)-benzotriazolyl acrylonitrile derivatives were synthesized under reflux conditions via Knoevenagel condensation between acetonitrile analogs and a short series of aromatic aldehydes. X-ray diffraction analysis for one of the compounds was performed to determine the (E)-geometry of its double bond. The photoluminescent properties of all compounds in solution were also evaluated. These compounds exhibit strong blue, green and yellow emission under ultraviolet light excitation with fluorescence quantum yield in the 0.08–0.58% range. To determine the suitability of these compounds for use in cell-based analysis, cytotoxicity assays were performed on a representative molecule using a common mammalian cell line (HEK 293T cells) at different concentrations. The results showed limited toxicity (72 ± 16% viability) at the highest concentration tested (50 μM). Finally, confocal microscopy demonstrated that our compound was internalized into cells and localized to endosomes and/or lysosomes in a similar fashion to Dextran–Cascade Blue (DCB).

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