Synthesis and characterization of amphotericin B stabilized gold nanoparticles sensor for detection of clindamycin drug

Abstract

Release of pharmaceuticals in water pose a major health threat to living organisms which demands development of a sensitive and selective method for detection of pharmaceutical drugs in water. In the present study, amphotericin B stabilized gold nanoparticles (APB-AuNPs) were successfully synthesized by chemical route, structural chracterization were carrited out by x-ray diffraction (XRD) and Fourier Transform Infrared (FT-IR) spectroscopy. Atomic force microscopy (AFM), scannining elecctron microscopy (SEM) and energy dispersive spectroscopy (EDX) were used to measure the surface morpholoy, elemental composition and average particle size distribution. Average particle size distribution was found to be in the range of 15–20 nm. The stability of synthesized gold nanoparticles was checked with respect to time and pH. APB-AuNPs showed stability at a pH range of 3–12 and remained stable for 18 days. The synthesized particles effectively detected clindamycin at 20 μM dose and the APB-AuNPs and clindamycin conjugate showed no interaction with other drugs in aqueous media. The intentional addition of Clindamycin in human blood plasma and tap water tends to shift and enhance the absorbance intensity of Amphotericin B stabilized gold nanoparticles (APB-AuNPs). Thus, providing a sensitive and selective tool for clindamycin detection in vitro. The present study will help in the development of new sensitive Amphotericin B stabilized gold nanoparticles (APB-AuNPs) based chemo-sensors for possible detection of Clindamycin even in the presence of other drugs.