Synthesis and biophysical characterization of porous bi-phase calcium phosphate/gelatin-PVA scaffold: Enhanced antibacterial, osteoconductivity and anticancer activity via silver nanoparticles and methotrexate incorporation

Abstract

In our relentless pursuit of advancing bone tissue regeneration, we have meticulously crafted multifunctional scaffolds that transcend traditional approaches. These freeze-dried scaffolds, composed of biphasic calcium phosphate (BCP) intricately woven into a gelatin-polyvinyl alcohol (PVA) matrix, harbor the potential to revolutionize regenerative medicine. The scaffolds are characterized with XRD, FTIR, SEM, 3D images of the SEM, mechanical properties, swelling and degradation tests, drug release, cell viability, antibacterial and anticancer tests. Our in-situ synthesis of BCP within the PVA framework yielded a biphasic structure comprising hydroxyapatite (HAp) and beta-tricalcium phosphate. These scaffolds exhibit good mechanical compressive strength (ranging from 28 to 173 kPa) and boast high porosity levels (approaching 80 %) enhance the processes of nutrition and waste removal. The incorporation of silver nanoparticles (Ag) introduces antimicrobial prowess, resulting in a complex scaffold texture characterized by well-defined pores and rugged walls. The interconnectivity within this fibrous network contributes to enhanced mechanical properties. Additionally, our scaffolds facilitate the sustained release of methotrexate (MTX), a potent anticancer agent. Within the first day, MTX is promptly delivered, potentially impacting cancer cells. These Ag-containing scaffolds effectively combat common pathogens, demonstrating significant antibacterial activity as an essential property for preventing infections at the implantation site. Notably, our scaffolds, whether infused with Ag or MTX (at a concentration of 0.25 wt%), exhibit no toxic effects on Vero and MG-63 cells within a 24-hour window. However, higher Ag content (2.0 wt%) shows cytotoxicity. In summary, our multifaceted scaffolds, poised at the intersection of science and creativity, hold immense promise for bone tissue engineering. Further investigations will explore long-term effects, broader microbial contexts, and in vivo models.