Abstract
A series of thiophene bearing pyrazolo[3,4-d]pyridazine and pyridine moieties were synthesized and characterized by IR, NMR, mass spectroscopic analytical techniques. In vitro antimicrobial activity of the synthesized compounds was screened against selected gram positive and gram negative bacteria, including Streptococcus pyogenes, Bacillus subtilis, Pseudomonas aeruginosa, and Escherichia coli as well as four fungi stains; Aspergillu niger, Syncephalastrum racemosum, Candida albicans, and Geotrichum candidum. Compounds 6 and 7 were found to be the highest active compounds against most of the strains. Compound 6 was found to be the most potent antibacterial agent compared to the standard drug Gentamicin against P. aeruginosa bacterial strain with growth inhibition value of 18.2 ± 0.77 to 17.3 ± 0.1. Compound 6 was then selected for further cytotoxic activity study against colon carcinoma (HCT-116) using the MTT assay method and a moderate cytotoxic activity against HCT-116 cell line was observed.
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