Synthesis and biological activities of polymers containingexo-3,6-epoxy-1,2,3,6-tetrahydrophthalic glycinyl imide

Abstract

The monomer, exo-3,6-epoxy-1,2,3,6-tetrahydrophthalic glycinyl imide(ETGI), was prepared by the Diels-Alder reaction of N-glycinylmaleimide and furan. Poly(ETGI), poly(ETGI-co-methacrylic acid)[poly(ETGI-co-MA)] and poly(ETGI-co-vinylacetate)[poly(ETGI-co-VAc)] were synthesized by photoinitiated homopolymerization of ETGI or copolymerizations of ETGI with MA and VAc. Synthesized ETGI, poly(ETGI), poly(ETGI-co-MA), and poly(ETGI-co-VAc) were characterized by IR and 1H-NMR spectroscopies, elemental analysis, and gel permeation chromatography. The in vitro cytotoxicities of ETGI, poly(ETGI), poly(ETGI-co-MA), and poly(ETGI-co-VAc) were evaluated using K-562 human leukemia cells and HeLa cells. In vitro cytotoxicity of monomer and polymers at a concentration of 1.0 mg/mL against K-562 human leukemia cells increased in the following order:poly(ETGI-co-MA) > poly(ETGI-co-VAc) > poly(ETGI) > Etgi. The cytotoxicities of copolymers against HeLa cells are less cytotoxic than ETGI at a dosage of 0.02, 1.0, and 5.0 mg/mL. The copolymers were very effective at any dosage tested. The in vivo antitumor activities of ETGI, poly(ETGI), poly(ETGI-co-MA), and poly(ETGI-co-VAc) were also evaluated against mice bearing sarcoma 180. In vivoantitumor activity of monomer and polymers at a dosage of 80 mg/kg increased in the following order: ETGI > poly(ETGI-co-VAc) > poly(ETGI-co-MA) > poly(ETGI) > 5-fluorouracil (5-FU).ETGI and polymers containing ETGI showed higher antitumor activity than 5-FU at any dosage tested. © 1996 John Wiley & Sons, Inc.