Synthesis and bioactivities of two multiple antigen peptides as potential vaccine against schistosoma

Abstract

Based on the two antigenic peptides, 26–43 (P 26) and 116–131 (P 116), derived from 28 kDa glutathione S-transferase of Schistosoma mansoni (Sm28GST), two multiple antigenic peptides (MAPs), (P 26) 4-MAP and (P 116) 4-MAP with the same oligomeric lysine core, were synthesized by stepwise solid-phase peptide synthesis method. The antigenicities and protective effects of these two MAPs were examined on experimental animals. As shown in the dot-ELISA result, the synthetic MAPs could be recognized and bound by immunoglobins in both patient’s and infected-rabbit’s sera. After Kunming mice were immunized with (P 26) 4-MAP, the worm burden reduction rate and the liver egg reduction rate were 59.9% and 61.1%. In (P 26) 4-MAP or (P 116) 4-MAP immunized BALB/c mice, the worm burden reduction rates were 37.5% and 62.5%, respectively, and the liver egg reduction rates were 35.1% and 54.0%, respectively.