Synthesis and bioactivities of silver nanoparticles capped with 5-Amino-ß-resorcylic acid hydrochloride dihydrate

Samina Nazir,Syed Alam,Muhammad Shah,Nazar Islam,Ajmal Khan,Sara Qaisar,Syeda Naz

doi:10.1186/preaccept-1660043240125066

Abstract

BackgroundConjugated and drug loaded silver nanoparticles are getting an increased attention for various biomedical applications. Nanoconjugates showed significant enhancement in biological activity in comparison to free drug molecules. In this perspective, we report the synthesis of bioactive silver capped with 5-Amino-?-resorcylic acid hydrochloride dihydrate (AR). The in vitro antimicrobial (antibacterial, antifungal), enzyme inhibition (xanthine oxidase, urease, carbonic anhydrase, ?-chymotrypsin, cholinesterase) and antioxidant activities of the developed nanostructures was investigated before and after conjugation to silver metal.ResultsThe conjugation of AR to silver was confirmed through FTIR, UV-vis and TEM techniques. The amount of AR conjugated with silver was characterized through UV-vis spectroscopy and found to be 9% by weight. The stability of synthesized nanoconjugates against temperature, high salt concentration and pH was found to be good. Nanoconjugates, showed significant synergic enzyme inhibition effect against xanthine and urease enzymes in comparison to standard drugs, pure ligand and silver.ConclusionsOur synthesized nanoconjugate was found be to efficient selective xanthine and urease inhibitors in comparison to Ag and AR. On a per weight basis, our nanoconjugates required less amount of AR (about 11 times) for inhibition of these enzymes.

Highlights

Conjugated and drug loaded silver nanoparticles are getting an increased attention for various biomedical applications
The in vitro antibacterial, antifungal, enzyme inhibition and antioxidant activities of Silver nanoparticles capped with AR (AgAR) nanoconjugates were compared with pure acid hydrochloride dihydrate (AR), Ag and the commercially available antibiotics, enzyme inhibitors and antioxidants
When the synthesized AR was added to the aqueous solution AgNO3, we observed a change in color from light brown to dark brown upon slow addition of NaBH4 (Additional file 1: Figure S1)

Summary

Introduction

Conjugated and drug loaded silver nanoparticles are getting an increased attention for various biomedical applications. Nanoconjugates showed significant enhancement in biological activity in comparison to free drug molecules In this perspective, we report the synthesis of bioactive silver capped with 5-Amino-β-resorcylic acid hydrochloride dihydrate (AR). The in vitro antimicrobial (antibacterial, antifungal), enzyme inhibition (xanthine oxidase, urease, carbonic anhydrase, α-chymotrypsin, cholinesterase) and antioxidant activities of the developed nanostructures was investigated before and after conjugation to silver metal. Silver is well-known for its antimicrobial activities and Current research showed that conjugation of Ag to plant extracts boosted the enzyme inhibition and antimicrobial activities but these reports are not very systematic [10,11] In this context the aim of our present work is to synthesize AgNPs from a synthetic biocide 5Amino-β-resorcylic acid hydrochloride dihydrate (AR). The in vitro antibacterial, antifungal, enzyme inhibition (xanthine oxidase, urease, carbonic anhydrase, α-chymotrypsin, cholinesterase) and antioxidant activities of AgAR nanoconjugates were compared with pure AR, Ag and the commercially available antibiotics, enzyme inhibitors and antioxidants

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Methods

Results

Conclusion