Synthesis and Bio-evaluation of Soluble Sodium Copper Chlorophyllin Complexes from the Leaves of Aloe vera

Abstract

Chlorophyllin is a semi-synthetic mixture of sodium copper complexes synthesized from chlorophyll. In the present investigation the copper-chlorophyllin (Cu-chlorophyllin) and soluble sodium copper chlorophyllin (Na-Cu-chlorophyllin) complexes were synthesized from leaves of Aloe vera. The synthesized compounds were estimated for the chlorophyll content. Na-Cu-chlorophyllin complexes showed high content of total chlorophyll (4.862 µg/mg). The synthesized compounds were characterized for its morphology by SEM, EDAX, FTIR and XRD. The complexes showed the presence of phytochemicals like phenols (21.62 µg/mL) and phytosterols (444.66 µg/mL) associated with it. The Na-Cu-chlorophyllin complexes showed antimicrobial activity with moderate results against S aureus, B cereus and maximum zone of inhibition against S typhi (23.6±0.33 mm).The complexes showed excellent free radical scavenging activity for DPPH with 61.84 % scavenging and IC50 value of 33.74 µg/mL. The complexes also exhibited moderate lethality of 30 % on brine shrimp after 72 h with an LC50 value 328.94 µg/mL. Since, Na-Cu-chlorophyllin complexes exhibited excellent bioactivity, it can be utilized extensively in various food, cosmetic, herbal medicine and pharma industries.