Abstract
Fluorescent silica nanoparticles incorporating unique ratios of energy-transfer dyes are synthesized and applied as colloidal barcodes to encode a microsphere-bound combinatorial peptide library. The affinity of the West Nile virus protease is profiled using this library with cleavage of the peptide detected by flow cytometry. The cleaved peptide substrates are sorted and then identified through decoding by confocal microscopy combined with spectral unmixing (see image).
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