Syntheses and Properties of Dansylated Deoxycytidine 3′,5′-Bisphosphate Derivatives Useful for 3′-Labeling of Oligoribonucleotides

Abstract

Abstract For non-RI labeling of RNAs with fluorescence markers, deoxycytidine 3′,5′-bisphosphate derivatives (1 and 2) were synthesized as dansyl donors which could be linked to the 3′-terminus of RNAs by T4 RNA ligase catalized joining reactions. Ligations of GpApC with these dansyl donors in the presence of T4 RNA ligase were studied.