Synergy of strains that accelerate biodegradation of pyridine and quinoline

Abstract

Three bacterial strains were isolated from activated sludge acclimated to biodegrade pyridine and quinoline simultaneously. The strains were identified as Bacillus tropicus, Bacillus aquimaris, and Rhodococcus ruber. When the isolated bacteria were used for pyridine and quinoline biodegradation in separate or combined modes, R. ruber had much faster kinetics, and combining R. ruber with one or both of the Bacillus strains increased further the biodegradation kinetics. For example, the time needed for complete biodegradation of 1 mM quinoline and pyridine decreased to 20 h and 6 h, respectively, with the three strains combined, compared to 26 h and 7 h with R. ruber alone. Whereas quinoline was completely mineralized by all three strains, 10–14% of the pyridine persisted as a dead-end product, 2-hydroxypyridine (2HP). The acclimated sludge from which the three bacterial species were isolated was able to transform 2HP, and adding the bacterial strains (especially R. ruber) to the acclimated sludge accelerated the rate of 2HP removal and mineralization through a form of synergy.