Synergy of bioactives from camelina seed meal for health promotion (647.16)

Abstract

Camelina sativa L. Crantz is under development as a novel oil seed crop yet there are few studies about the bioefficacy of camelina. Camelina seed meal contains several potentially bioactive compounds: two major aliphatic GSLs, glucoarabin (9‐(methylsulfinyl)nonylglucosinolate; GSL9), glucocamelinin (10‐(methylsulfinyl)decylglucosinolate; GSL10), with trace amount of a third, 11(methylsulfinyl)undecylglucosinolate (GSL11) and several flavonoids, mostly as quercetin glycosides. We tested hydrolyzed glucosinolates (hGSL) and flavonoids quercetin and rutin for their ability to upregulate detoxification enzymes, as biomarkers of anti‐cancer activity. None of these compounds either caused induction of CYP1A1 activity, or inhibition of β‐naphthoflavone‐induced CYP1A1 activity. In contrast, hGSL9 and hGSL10, quercetin and rutin alone and in combination all caused induction of quinone reductase (NQO1) activity. We found synergistic induction of NQO1 activity when Hepa1c1c7 cells were treated with a combination of all the four major bioactives in the proportions present in camelina seed extract. Although potency of hGSL9 was somewhat less than that of sulforaphane, we found that GSL9 and quercetin appear responsible for this synergy. As Camelina seed extract possesses NQO1 inducing activity, it should be evaluated for anticancer activity, similar to other Brassicaceae family members, such as Broccoli.Grant Funding Source: Supported by USDA/NIFA #AG 2010‐65200