Abstract
Organoids have emerged as a groundbreaking technique in biomedical research. Leveraging clinical samples, they enable the rapid creation of in vitro models that faithfully preserve the pathological, genetic, and epigenetic features of patient samples, thereby illuminating preclinical research. CRISPR, a well-established technique, empowers precise genetic manipulation on a large scale. The combination of these techniques enables researchers to simultaneously manipulate thousands of genes and assess their impact on phenotypes. In this study, we analyze several papers that integrate organoids and CRISPR techniques to identify key factors in development or pathogenesis.
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