Synergistic role of pipronyl butoxide in improving the Spodoptera littoralis nucleopolyhedrosis virus effectiveness against Spodoptera littoralis and studying its histopathological biosafety

doi:10.35495/ajab.2021.05.227

Abstract

Highlights

The Egyptian cotton leaf worm, Spodoptera littoralis (Lepidoptera: Noctuidae) is the most destructive, serious and widespread pest in Egypt and in subtropical and tropical regions (Elmenofy et al, 2020)
Rats treated with 0.3% pipronyl butoxide (PBO), or 0.3% PBO plus Spodoptera littoralis nucleopolyhedrosis virus (SlNPV) revealed a significant increase in Caspase-3 immunoexpression in the cytoplasm of both hepatic and renal cells as indicated by dark brown color when compared with all other groups
Proliferating cell nuclear antigen (PCNA) Immunoexpression Liver and kidney tissues stained for PCNA antibodies of both normal control group and SlNPV treated groups showed limited immune reaction in hepatocytes, while PCNA-positive cells were not found in the kidney sections

Summary

Introduction

The Egyptian cotton leaf worm, Spodoptera littoralis (Lepidoptera: Noctuidae) is the most destructive, serious and widespread pest in Egypt and in subtropical and tropical regions (Elmenofy et al, 2020) It is known as polyphagous insect as it infests and attack various economically important crops over the year causing economical yield losses in these crops (Mohamed et al, 2019). The excessive use of chemical insecticides leads to harmful effect on natural enemies and all other non-target insect, they cause detrimental problems to humans, plant and animals and induce negative impact to environment. Baculoviruses are the largest and most widely insect specific entomopathogens belonging to family Baculoviridae, they are promising alternatives to decrease dependency on deleterious chemical insecticides They have greatest potential for use as microbial insect pest control agents against several insect pest (Lacey et al, 2015). Synergists have a very fundamental role in increasing the efficacy of insecticide as they inhibit the natural defense of the insects by altering the insecticide detoxification enzymes (Moores et al, 2009)

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