Synergistic modes of interaction between the plant essential oils and the respiratory blocker chlorfenapyr

Abstract

Plant essential oils are widely acknowledged for their insecticidal activities and synergistic interaction with conventional insecticides, but their insecticidal modes of action and the mechanism of synergy remain less understood. In this study, electrophysiological screenings on the larval central nervous system (CNS) of the common fruit fly, Drosophila melanogaster, and the housefly, Musca domestica, were conducted to identify the neurophysiological effects of the oils and their major constituents. Several oils changed the firing rate of the central motor neurons, and four oils were selected to determine their major active compounds. Eugenol and thymol (87.2% and 31.1% in clove bud and thyme oils, respectively) were inhibitory to the nerve firing rates of the CNS, and exhibited synergistic toxicity to the housefly when blended with a respiratory blocking pyrrole insecticide, chlorfenapyr. On the other hand, trans-cinnamaldehyde and terpinen-4-ol (74.6% and 52.0% in cinnamon and teatree oils) seemed excitatory to the nerves, and displayed antagonistic interaction to chlorfenapyr in their insecticidal activity. Chlorfenapyr led to ATP depletion in the insects, and the inhibitory compounds accelerated the process. On the other hand, nerve-excitatory compounds seemed to nullify the depletion. This was further confirmed with the two CNS-excitatory synthetic insecticides, permethrin and chlorpyrifos, that they exhibited antagonistic toxicity when mixed with chlorfenapyr. Meanwhile, the synergy between the inhibitory compounds and chlorfenapyr was diminished when ATP was artificially injected, indicating that the bioenergetic effects of neuroinhibitors are responsible for their synergistic interactions.