Abstract
The aim of this study was to determine the synergistic effect of nisin and green onion extract (GOE) on the inhibition of Listeria monocytogenes cocktail found in full-fat milk (M1), fat-free milk (M2), nano-calcium milk (M3) and nano-iron milk (M4). GOE at concentrations of 2, 5 and 8% revealed significant antilisterial effect against L. monocytogenes cocktail in all retail milk. The addition of nisin at concentrations of 75, 150, 225 and 300 IU/mL also showed a good antilisterial effect compared with the control group. Combination of GOE (2 and 5%) and nisin (75, 150, 225 and 300 IU/mL) resulted in synergistic antilisterial activity in all samples tested (M1∼M4) at 14 days. The synergistic antilisterial ability shows the potential application of combining GOE and nisin as an antilisterial agent in the food industry. PRACTICAL APPLICATIONS Our study shows the synergistic effect of nisin and GOE on the inhibition of L. monocytogenes cocktail present in various retail milk samples including M1, M2, M3 and M4. Together, GOE and nisin can be a potential antilisterial agent for the food additive application in the food industry.
Published Version
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