Abstract
Abstract This study assessed the effect of the combined application of carvacrol (CAR) and 1,8-cineole (CIN) against bacteria associated with minimally processed vegetables (MPV), namely Listeria monocytogenes, Aeromonas hydrophila and Pseudomonas fluorescens, in mixed culture. For this, Fractional Inhibitory Concentration (FIC) index, time-kill in vegetable-based broth, vegetable matrices and confocal and scanning electron microscopy analysis were carried out. CAR and CIN displayed Minimum Inhibitory Concentrations (MIC) of 1.25 and 40 μL/mL, respectively. FIC index of the combined compounds was 0.25 against the mixed inoculum, suggesting a synergic interaction. The application of CAR and CIN alone (MIC) or combined (1/8 MIC + 1/8 MIC) in vegetable-based broth caused a decrease (p
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