Synergistic effects of essential oils and phenolic extracts on antioxidant activities responses using two Artemisia species (A. campestris and A. herba alba) combined with Citrus aurantium

Abstract

The aim of the present work was to investigate the synergistic effects of the binary and the ternary combinations of extracts using the following plants: A. campestris (AC), A. herba alba (AHA) and C. aurantium (CA). Antioxidant activities of essential oils (EO) were determined using DPPH and β-carotene assays, whereas, antioxidant activities of phenolic extracts (PE) were determined using DPPH, FRAP and ABTS assays. The antioxidants activities of the extracts combinations were evaluated using the Simplex Lattice Mixture Design. For the EOs, the results showed the existence of a very interesting dilution effect of CA for AC and AHA EOs (up to 1/3 vol fraction for DPPH and up to 1/2 for β-carotene). Alternatively, interesting synergistic effects were recorded in the following area of interest for the PEs “0.15 < AC<0.6 & AHA<1/3 & 1/3 < CA<2/3”. The synergistic effects was observed for the three tested antioxidant activities (DPPH, FRAP and ABTS).